Part:BBa_K2057008:Design
Alginate lyase and microcin controlled by lasI promoter
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
No consideration
Source
Alginate lyase come from the chromosome of Bacillus thuringiensis 4Q7. LasI promoter and microcin are harbored in the biobricks BBa_K649000 and Bba_K565004, respectively.
References
(1) Farrel EK, Tipton PA (2012) Functional characterization of AlgL, an alginate lyase from Pseudomona aeruginosa. Biochem. 51:10259-10266. (2) Saeidi N, Wong CK, Lo TM, Nguyen HX, Ling H, Leong SS, Poh CL, Chang MW (2011) Engineering microbes to sense and eradicate Pseudomonas aeruginosa, a human pathogen. Mol Syst Biol. 7:521 (3) Barboza Corona JE, Nieto-Mazzocco E, VelĂĄzquez-Robledo R, Salcedo-Hernandez R, Bautista M, JimĂ©nez B, Ibarra JE (2003) Cloning, sequencing and expression of the chitinase chiA74 from Bacillus thuringiensis. Appl Environ Microbiol. 2003 Feb;69(2):1023-1029. (4) ZschĂŒttig A, Zimmermann K, Blom J, Goesmann A, Pöhlmann C (2012) Identification and characterization of Microcin S, a new antibacterial peptide produced by probiotic Escherichia coli G3/10. PLoS ONE 7(3): e33351. doi:10.1371/journal.pone.0033351